Fig. 1.

Flow cytometry of ASC markers. Representative flow cytometry readouts for ASC surface marker expression of CD73 (a), CD105 (b), and CD90 (c) for both burned human (BH) and unburned human (UH) patients. The bottom row shows quantification, which shows that while there were no differences in any marker at any passage between BH and UH, there was a significantly lower expression of each marker at passage 2, compared to subsequent passages. Expression of CD73 on UH ASCs was 70.8 ± 9.8, 95.8 ± 3.9, and 97.6 ± 1.3% at passages 2, 4, and 6, respectively, while the expression on BH ASCs was 64.0 ± 8.6, 99.7 ± 0.1, and 99.2 ± 0.4%, respectively. Expression of CD105 on UH ASCs was 52.3 ± 11.7, 83.8 ± 12.9, and 92.9 ± 3.2% at passages 2, 4, and 6, respectively, while the expression on BH ASCs was 47.4 ± 8.2, 94.6 ± 1.8, and 89.5 ± 4.4%, respectively. Expression of CD90 on UH ASCs was 75.3 ± 5.8, 94.4 ± 5.4, and 98.5 ± 0.8% at passages 2, 4, and 6, respectively, while expression on BH ASCs was 69.8 ± 10.5, 99.5 ± 0.3, and 99.2 ± 0.2%, respectively. *P < 0.05, **P < 0.01, ***P<0.001. N = 6 patients in BH ASCs and UH ASCs at each passage