Figure 3.

sDR5-Fc suppressed the release of IL-1β and TNF-α in M1 macrophages.

All samples were simulated with LPS + IFN-γ for 24 h firstly and then treated with different concentrations of sDR5-Fc. With the growing of sDR5-Fc, IL-1β and TNF-α were suppressed in mRNA (A) and protein level (B). Data are expressed as mean ± SD from at least three representative experiments. ^*P < 0.05, ^**P < 0.01, compared with the group of sDR5-Fc and LPS + IFN-γ with indicated concentration of 0 μg/mL. IFN-γ: interferon-gamma; IL-1β: interleukin-1β; LPS: lipopolysaccharide; sDR5-Fc: soluble death receptor 5-Fc; TNF-α: tumor necrosis factor-α.