Figure 4.

sDR5-Fc suppressed the glycolysis of M1 macrophages.

All samples were simulated with LPS + IFN-γ for 24 h firstly and then treated with different concentrations of sDR5-Fc. With the growing concentration of sDR5-Fc, pH of culture medium was rising to neutral (A) while the concentrations of lactic acid were reduced (B). sDR5-Fc decreased the mRNA expression of HK2 and GLUT1 in the M1 macrophages with a concentration-dependent manner (C) and time-dependent manner (D). Also, sDR5-Fc decreased the protein expression of HK2 and GLUT1 in the M1 macrophages with a concentration-dependent manner (E & F) and time-dependent manner (G & H). Data are expressed as mean ± SD from at least three representative experiments. ^*P < 0.05, ^**P < 0.01, compared with the group of sDR5-Fc and LPS + IFN-γ with indicated concentration of 0 μg/mL. GLUT1: glucose transporter 1; HK2: hexokinase 2; IFN-γ: interferon-gamma; LPS: lipopolysaccharide; sDR5-Fc: soluble death receptor 5-Fc.