Figure 5.

Knock-down of DR5 inhibited the glycolysis of M1 macrophages.

(A): DR5-siRNA significantly reduced the elevated pH induced by LPS + IFN-γ; (B): DR5-siRNA reduced the elevated expression of mRNA of HK2 and GLUT1 induced by LPS + IFN-γ; and (C & D): DR5-siRNA reduced the elevated expression of HK2 and GLUT1 proteins in M1 macrophages. Data are expressed as mean ± SD from at least three representative experiments. ^*P < 0.05, ^**P < 0.01, compared with the control group. DR5: death receptor 5; GLUT1: glucose transporter 1; HK2: hexokinase 2; IFN-γ: interferon-gamma; LPS: lipopolysaccharide; sDR5-Fc: soluble death receptor 5-Fc; siRNA: small interfering RNA.