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. 2020 Aug 21;34(5):867–879. doi: 10.5713/ajas.20.0115

Table 1.

Primers used to amplify FN3, GH5 genes of the gene XFN3GH5 encoding mature endoglucanase XFN3GH5

Primers Sequence (5′-3′)
Fn3f TCCATGGCGACCAACCCGGCGAAGGTTAC
Fn3r TCTCGAGGGTCTTAACGGTCACGTTATCGC
Gh5f TCCATGGTTAACCAGAGCGGTCAAATTTTC
Gh5r TCTCGAGGTCGGTGCCGGTCTTAAACG

Underlined sequences indicated restriction sites for cloning gene into pET22b(+) and pETSUMO.