Table 1.
Primers | Sequence (5′-3′) |
---|---|
Fn3f | TCCATGGCGACCAACCCGGCGAAGGTTAC |
Fn3r | TCTCGAGGGTCTTAACGGTCACGTTATCGC |
Gh5f | TCCATGGTTAACCAGAGCGGTCAAATTTTC |
Gh5r | TCTCGAGGTCGGTGCCGGTCTTAAACG |
Underlined sequences indicated restriction sites for cloning gene into pET22b(+) and pETSUMO.