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. 2020 Oct 13;34(6):957–966. doi: 10.5713/ajas.20.0392

Figure 2.

Figure 2

miR-458b-5p directly regulates CTNNB1. (A) Schematic diagram of the dual luciferase reporter pmir-GLO-CTNNB1-3′UTR. (B) Dual luciferase assays were performed by co-transfection of miR-458b-5p mimics or mimics NC and wild-type vectors or mutant vectors. (C) The relative CTNNB1 mRNA expression levels after treatment with the miR-458b-5p mimics. (D) Western blot analysis of β-catenin protein expression after treatment with the miR-458b-5p mimics. (E) The quantification of β-catenin protein levels. CTNNB1, catenin beta-1; WT, wild-type; MT, mutant-type; UTR, untranslated regions; MCS, multiple cloning site; NC, negative control. Results are presented as means±standard error of the mean of three independent determination, ** p<0.01. Bars with different letters are significant different (p<0.05).