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. 2020 Oct 13;34(6):957–966. doi: 10.5713/ajas.20.0392

Figure 3.

Figure 3

miR-458b-5p inhibits the proliferation of chicken ovarian GCs. (A) Transfection with the miR-458b-5p mimics remarkably increased the miR-458b-5p expression level after transfection 48h. (B) Chicken ovarian GCs proliferation estimated by CCK8 assay in response to overexpression of miR-458b-5p after transfection 0, 12, 24, 36, 48, and 60 h. (C) Cell cycle was detected in chicken GCs 2 days after transfection. (D) Histogram represented the percentage of cells in the G1, S, and G2 phases. GCs, granulosa cells; CCK-8, cell counting kit-8. * p<0.05.