Table 1.

Primer sequences

Genes	Primers	Annealing (°C)
CTNNB1	Forward primer: 5′-CCGAAACACTGGATGAAGGA-3′ Reverse primer: 5′-GCTGATGAACCATAACCGCA-3′	54
BIRC5	Forward primer: 5′-GAATGGCTGGTCTACCTCGT-3′ Reverse primer: 5′-CACCGTCAGGTTAGAGGGAT-3′	56
CD44	Forward primer: 5′-ATCAGGGACCACACAAGGGA-3′ Reverse primer: 5′-ACTTGCTGGCATCTCCGTTT-3′	60
MMP7	Forward primer: 5′-GTTACCTCGGGACAGGCAGA-3′ Reverse primer: 5′-CAGGGCTCCACGGACATTTG-3′	60
GAPDH	Forward primer: 5′-GCTGATGCTCCCATGTTCGTGAT-3′ Reverse primer: 5′-GTGGTGCAAGAGGCATTGCTGAC-3′	61
miR-458b-5p	Forward primer: 5′-AGTACCATTCAAAGAGCTATGA-3′	60

CTNNB1, catenin beta-1; BIRC5, baculoviral IAP repeat containing 5; CD44, CD44 molecule; MMP7, matrix metallopeptidase 7; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.