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. 2020 Aug 24;34(6):1006–1013. doi: 10.5713/ajas.20.0349

Figure 1.

Figure 1

Effect of different doses of 5-ALA on bovine MEC viability. Confluent bovine MECs were treated with 50, 100, 250, and 500 μM of 5-ALA for 48 h. Cell viability was measured using MTT assays. Absorbance was measured at 570 and 630 nm using a multimode microplate reader to calculate survival rates expressed as the percentage of the control cells without 5-ALA treatment. Data are presented as mean± standard error of the mean for three independent experiments. 5-ALA, 5-aminolevulinic acid; MEC, mammary epithelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. * Indicates p<0.05 compared with the control.