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. 2020 Aug 24;34(6):1006–1013. doi: 10.5713/ajas.20.0349

Figure 2.

Figure 2

5-ALA counters HS reduced cell viability in bovine MECs. (A) Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 48 h). Cell viability was measured by MTT assay and expressed the percentage of control cells continuously cultured at 37°C and received no 5-ALA treatment. (B) Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 48 h). mRNA levels of BAX and BCL2 were determined by RT-qPCR and normalized to ACTB levels. Data are presented as mean±standard error of the mean for three independent experiments. 5-ALA, 5-aminolevulinic acid; HS, heat stress; MEC, mammary epithelial cells; RT-qPCR, real-time quantitative polymerase chain reaction; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. a–c Means with different letters are significantly different, p<0.05.