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. 2020 Aug 24;34(6):1006–1013. doi: 10.5713/ajas.20.0349

Figure 4.

Figure 4

5-ALA reduces HS induced ER stress. Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 24 h). Cells that were consistently cultured at 37°C and received no 5-ALA treatment were used as the control group. (A) mRNA levels of GRP78, CHOP, and XBP1s were determined by RT-qPCR and normalized to ACTB levels. Data are presented as mean± standard error of the mean for three independent experiments. a–c Means with different letters are significantly different, p<0.05. (B) The phosphorylated PERK, PERK, CHOP, cleaved caspase3, and α-tubulin (internal control) protein levels were detected using Western blotting. Representative images from three independent experiments with at least four replicates in each experiment are shown. 5-ALA, 5-aminolevulinic acid; HS, heat stress; ER, endoplasmic reticulum; MEC, mammary epithelial cells; GRP78, glucose-regulated protein 78; CHOP, C/EBP homologous protein; XBP1s, X-box binding protein 1 splicing form; PERK, PKR-like endoplasmic reticulum kinase.