Table 1.

Properties of SARS-CoV-2 Spike Proteins.

Name	Descriptor		ΔTotalEnergy	ΔDisulfidePotential	ExpressionImpact	Trimer Formation
Spike Monomer	No trimerization domain				++++	–
Spike_Furin	Full furin Site				+++	+
		Controls
Spike_PP	K986P/V987P; Knocked out furin site				+++	+
Spike_KV	Knocked out furin site				++	+
RCC1	V382C - R983C		4.53	-0.42	–	Undetermined
RCC2	A520C- K41C		4.76	0.37	–	Undetermined
RCC3	S383C - D985C		6.17	1.02	++	+
RCC4	D614C - T859C		7.29	0.83	+	+
RCC5	T547C- N978C	Cross-linking Designs	8.84	0.88	+	+
RCC6	A570C - V963C		8.96	3.08	+++	+
RCC7	P665C - L864C		9.07	0.82	–	Undetermined
RCC8	F562C - P225C		9.52	0.14	–	Undetermined
RCC9	P589C - F855C		9.74	1.48	–	Undetermined

Table includes the shorthand name of different Spike elements explored here, the descriptor of each design, Talaris 2014 energies, the dslf_fal3 energy term, relative expression impact (denoted as -,+ > 0 µg/L, ++ > 100 µg/L, +++ > 500 µg/L, or ++++ > lOOOµg/L), and trimer fmmation (denoted as +, -, or undetennined).