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. 2021 Apr 22;12:666334. doi: 10.3389/fphar.2021.666334

FIGURE 3.

FIGURE 3

β3AR stimulation accelerates reperfusion following hind limb ischemia. (A) Representative images showing Laser Doppler flux in hindlimbs from mice immediately post-ligation and after 14 days of recovery, treated with vehicle (saline) or CL 316,243 (1 mg/kg/day) (B) Summary data of hind limb perfusion both pre- and post-ligation and in the contralateral control limb shown as raw flux data. (C) Calculated ratio of the ischemic to non-ischemic limbs following 14 days of hind limb ischemia. (D) eNOS activity by radioimmunoassay in hind limb tissue from mice treated with vehicle or CL 316,243, at 14 days post-surgery. (E) Superoxide generation measured by lucigenin-enhanced chemiluminescence (20 μmol/L) corrected for background luminescence. (F) Immunoblot expression of hindlimb Nox 2 and Nox 4 with β-actin control, V = vehicle, CL = CL-316,243-treated. Mean ± SEM; *p < 0.05 vs vehicle by 1-way or 2-way ANOVA with Bonferroni post-hoc analysis; n = 8. +++ p < 0.001 vs. Pre-ligation limb perfusion; ### p < 0.001 vs. post-ligation (timepoint 0) reperfusion.