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. 2021 Apr 22;12:666334. doi: 10.3389/fphar.2021.666334

FIGURE 6.

FIGURE 6

Glutathionylation of eNOS (eNOS-GSS) in ischemic hind limb samples from type 1 (T1) diabetic mice. (A) Representative images of immunoblots (IB) performed on protein fractions following eNOS immunoprecipitation (IP). On the left the expression of GSH, detected at 680 nm is shown and in the middle, the simultaneous expression of eNOS, detected at 800 nm are shown. The right panel shows the merged image of both detection channels. The negative control, -IgG antibody used during IP, is shown only in the top panel. All samples were extracted and run simultaneously. (B) Summary data of eNOS glutathionylation shown as the ratio of glutathionylated eNOS to total eNOS in ischemic hind limb samples. (C) eNOS relative to β-actin expression in total ischemic hindlimb lysate from immunoblot. (D) phosphorylated eNOS (serine 1177) relative to total eNOS in the ischemic hindlimb. Summary data presented as mean ± SEM. Statistical analysis by 1-way ANOVA with Bonferroni post-hoc analysis. ## p < 0.01 vs. citrate, *p < 0.05 vs. vehicle.