FIGURE 6.

TBCA is present in a high amount in mouse insoluble protein extracts from the heart. Soluble (A) and insoluble (B) protein extracts from mouse organs (heart, brain, kidney, liver, lung, and testis) were prepared and analyzed on 16.5% (w/v) Tricine–SDS–PAGE followed by western blot with antibodies against TBCA, α-tubulin, and β-actin. Soluble protein extracts were quantified, and equal protein amounts (100 μg) were analyzed. Insoluble protein extracts were solubilized with 8 M urea and diluted 1:2 in loading buffer and, 20 out of 200 μl were analyzed. (A) The analysis showed that TBCA was more abundant in testis and brain. α-tubulin and β-actin are detected in all organs. (B) The insoluble protein extracts show that TBCA is more abundant in the heart and testis. α-tubulin is mainly detected in testis and brain, and β-actin is detected in all organs. The approximate molecular mass of the proteins is indicated on the left side of the panels.