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. 2021 Apr 22;9:642605. doi: 10.3389/fcell.2021.642605

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Copyright © 2021 Xu, Yao, Ni, Zheng, Huang, Huang, Gao, Qiao, Shen and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing circSIPA1L1 attenuated cell proliferation, invasion, and migration in OS cells. (A) The expression levels of circSIPA1L1 and SIPA1L1 mRNA in 143B and HOS cells after stably transfected with N.C. (negative control) or sh-circSIPA1L1 were evaluated by qRT-PCR. (B) Migration-related experiments confirmed circSIPA1L1 knockdown to suppress cell migration and invasion abilities of 143B and HOS cells. (C) Wound-healing assay was used to determine cell migration capacity of the stable 143B and HOS cells. (D) shRNA-mediated circSIPA1L1 knockdown attenuated OS cell growth, as confirmed by CCK-8 experiments in 143B and HOS cells. (E) Colony formation assay was performed to measure the cell growth capacity of transfected 143B and HOS cells. (F) Both 143B and HOS cells were transfected with sh-circSIPA1L1, followed by Annexin V-FITC/PI staining. Data from 3 independent experiments are presented as mean ± SD. *P < 0.05.