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. 2021 Apr 22;9:642605. doi: 10.3389/fcell.2021.642605

FIGURE 3.

FIGURE 3

circSIPA1L1 functioned as a miR-411-5p sponge in OS progression. (A) The expression levels of circSIPA1L1 in circSIPA1L1 knockdown 143B cells were determined by RIP assays. (B) Schematic illustration presenting overlapping of the downstream miRNAs of circSIPA1L1 predicted by Targetscan, miRanda, and RNAhybrid. (C,D) qRT-PCR detected the relative miRNA levels of 16 miRNA candidates in the 143B and HOS lysates. (E) Luciferase reporter assays were employed to measure the luciferase activities of 293T cells co-transfected with mimic N.C. or miRNA mimics. (F) Apoptosis assay showed the apoptotic percentage of 143B and HOS cells transfected with mimic N.C. or miRNA mimics. (G) Schematic illustration depicting that circSIPA1L1 shares a miRNA response element with miR-411-5p. (H) 293T cells were co-transfected with miR-411-5p mimics (or mimic N.C.). Data from three independent experiments are presented as mean ± SD. *P < 0.05.