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. 2021 Apr 22;9:642605. doi: 10.3389/fcell.2021.642605

FIGURE 5.

FIGURE 5

RAB9A serves as a target gene of miR-411-5p. (A) Schematic illustration exhibiting overlapping of the downstream genes of miR-411-5p, as determined by TargetScan, miRDB, and miRWalk. (B) qRT-PCR was utilized to evaluate the expression of five selected genes in OS cells transfected with sh-circSIPA1L1 or N.C. (C) qRT-PCR was conducted to assess the expression of RAB9A in OS cell lines. (D) Schematic illustration exhibiting the complementary sequence between miR-411-5p and RAB9A. (E) 293T cells were co-transfected with miR-411-5p mimics (or mimic N.C.). (F) The mRNA level of RAB9A in OS cells transfected with miR-411-5p mimic or miR-411-5p inhibitor was detected using qRT-PCR. (G) Western blotting was employed to evaluate the capacity of miR-411-5p to influence the expression of E-cadherin, N-cadherin, and RAB9A at the protein level. Data from three independent experiments are presented as mean ± SD. *P < 0.05.