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. 2021 Apr 22;9:642605. doi: 10.3389/fcell.2021.642605

FIGURE 6.

FIGURE 6

RAB9A functions as an oncogene in OS. (A) qRT-PCR demonstrated that overexpression of RAB9A could restore the decrease of RAB9A mRNA expression induced by sh-circSIPA1L1 in 143B and HOS cells. (B) Expression of N-cadherin, E-cadherin, and RAB9A at the protein level in 143B and HOS cells was determined using western blotting. Cells were transfected with vector or sh-circSIPA1L1, with or without RAB9A overexpression. (C) Cell migration and invasion abilities of OS cells transfected with control vector or sh-circSIPA1L1, with or without RAB9A overexpression, were determined by migration and invasion assays. (D) RAB9A overexpression accelerated the migration of sh-circSIPA1L1-mediated cells, as determined by a wound-healing assay. (E) The effects of circSIPA1L1 knockdown and RAB9A overexpression on cell vitality were determined by a CCK-8 assay. (F) The proliferation capacity of stable 143B and HOS cells was detected by a colony formation assay. (G) Apoptosis assay demonstrated the alteration of cell survival ability of 143B and HOS cells under the effects of circSIPA1L1-deficiency and RAB9A-overexpression. Data from three independent experiments are presented as mean ± SD. *P < 0.05.