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Variation of lifetimes of Mac-1–GPIbα bonds versus force. All data were from single molecular AFM measurements and shown as mean ± SEM. At least 325 single-bond rupture force data at each group were collected and analyzed using a force bin of 7.5 pN. Cantilever tip and petri dish were functionalized through coating with 15 μg/ml Mac-1 and GPIbα, respectively (Materials and Methods).
