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. 2021 Apr 22;8:635013. doi: 10.3389/fvets.2021.635013

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Copyright © 2021 Wang, Zhou, Ali, Zhang, Wang, Huang, Luo, Zhang, Qin, Zhang, Zhang, Zhou and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Volcano plot and clustering analysis of differentially expressed PIWI-interacting RNAs (DE piRNAs) in fresh and frozen-thawed giant panda and boar sperm. (A) Volcano plot of DE piRNAs in fresh and frozen-thawed giant panda sperm. Blue dots represent normal expressed, green dots represent the downregulated, and red dots represent the upregulated piRNAs. (B) Heat maps of the cluster analysis of piRNAs. Red indicates high expression while green means low expression of piRNAs.