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ChIP Final Wash Buffer 500 mL
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 500 mM | 5 M NaCl 50 mL |
| EDTA pH8.0 | 2 mM | 0.5 M EDTA 2 mL |
| Triton X-100 | 1% | Triton X-100 5 mL |
| SDS | 0.1% | SDS 0.5g |
| Tris-HCL | 20 mM | 1 M Tris-HCL 10 mL |
The buffer can be stored at 4°C for six months.
