| Sample | Volume |
|---|---|
| Template DNA | 1 μL |
| 2∗SYBR SYBR Green Master mix | 10 μL |
| Primers (forward, 10 μM) | 1 μL |
| Primers (reverse, 10 μM) | 1 μL |
| ddH2O | 7 μL |
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