Figure 8.

HeLa cells exhibit prolonged metaphase delay and spindle positioning defects in the presence of native-like n-ZPrC-RFP670

Stable-transfected GFP-tubulin (green) HeLa cells were transiently transfected with mScarlet-H2A (red) and n-ZPrC-RFP670 (cyan). Timelapse confocal data were collected 24 h post-transfection. Also visible in the field of view (lower right) is a mitotic cell that is co-transformed for GFP-tubulin and mScarlet H2A, but with no detectable n-ZPrC-RFP670 fluorescence, and which divides with normal timing and stable spindle orientation. Scale bar, 25μm, time = mm:ss.