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. 2021 Mar 27;18(11):2251–2261. doi: 10.7150/ijms.56024

Figure 6.

Figure 6

GRP94 regulates ETV1 expression through MAPK pathway. (a)The mRNA level of ETV1 was detected by QPCR and was dramatically decreased after treating p38 and ERK inhibitor in DLD-1 cells, while the level of ETV1 was remained unchanged after treatment of JNK inhibitor (n=3). (b) The protein level of ETV1 was determined by western blot and was decreased after treatment of p38(3μM), ERK (50μM), and JNK (50μM) inhibitors (n=3). (c) Protein levels of p38/p-p38, JNK/pJNK, and ERK/pERK were significantly decreased after treatment of p38, JNK, and ERK inhibitors by western blot (n=3). (**p<0.01, ***p<0.001. ****p<0.0001) (d) Cytotoxicity of the inhibitors were determined by CCK8 assay after treating the cells with p38(3μM), ERK (50μM), and JNK (50μM) inhibitors (n=3).