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. 2021 Apr 9;18(11):2355–2365. doi: 10.7150/ijms.52643

Figure 4.

Figure 4

(A) CCK-8 results of FA-MNP MNP-CDDP FA/TFPI-2 and FA-MNP/CDDP/TFPI-2 at different concentrations on HEN-1 cells. (B) Apoptosis assay (a: FA-MNP group; b: MNP-CDDP group; c: FA-TFPI-2 group; d: FA-MNP/CDDP/TFPI-2 group). (C) Cell cycle analysis (a: FA-MNP group; b: MNP-CDDP group; c: FA-TFPI-2 group; d: FA-MNP/CDDP/TFPI-2 group). (D) In vivo MRI nasopharyngeal carcinoma-bearing nude mice [HNE-1 (right tumor) CNE-2 (left tumor)] (T1: T1-weighted; T2: T2-weighted). (E) In vivo imaging nasopharyngeal carcinoma-bearing nude mice (Left: HNE-1 nude mouse models; Right: CNE-2 nude mouse models, 48 h: administrated with FA-MNP/CDDP/TFPI-2 for 48 h). (F) Tunel staining of tumor tissue sections obtained from mice models 28 days after the final treatment. a: FA-MNP groups; b: FA-TFPI-2 group; c: MNP-CDDP group; d: FA-MNP/CDDP/TFPI-2 group. Positive signals in TUNEL staining were developed by DAB. Cell nuclei were counterstained with hematoxylin. Sepia in Tunel staining indicated apoptosis or necrosis, respectively.