Figure 3.

Aged mice have distinct T_reg phenotype and elevated levels of IL-1β compared to young mice. 16 weeks after 4-NQO administration, single-cell suspension from tongue was re-stimulated with PMA/Ionomycin for 4 hours. Flow cytometry histograms showing indicated protein expression in CD45⁺ CD3⁺ CD4⁺Foxp3⁺cells (A). Tongue tissues were processed for IL-1β immuno-histochemical staining at 5 weeks (B). Supernatants collected at 12 weeks were processed for IL-1β ELISA. Mean +/- SEM in 3 mice/group (Mann-Whitney test *P< 0.05) (C). Tongue tissues were processed for IL-1β immuno-histochemical staining at 24 weeks (D).