TABLE 1.
Prophylactic treatment with RRx-001 Reduced Pancytopenia after Sublethal Irradiation.
| Time after radiation | Treatment group | WBC (×103 cells/µL) | ALC (×103 cells/µL) | ANC (×103 cells/µL) | Platelets (×103 cells/µL) | % Hematocrit | % Reticulocytes |
|---|---|---|---|---|---|---|---|
| Average days 2, 7, 14, 21, 28 | Vehicle + sham | 5.01 ± 0.31 | 3.75 ± 0.26 | 0.91 ± 0.06 | 913.66 ± 52.55 | 41.59 ± 0.44 | 2.54 ± 0.07 |
| RRx-001 + Sham | 4.73 ± 0.27 | 3.38 ± 0.21 | 0.97 ± 0.06 | **1,067.73 ± 32.26 | 41.66 ± 0.45 | 2.62 ± 0.07 | |
| Day 2 | Vehicle +7 Gy | 0.82 ± 0.14 | 0.07 ± 0.01 | 0.67 ± 0.13 | 863.00 ± 88.08 | 39.27 ± 0.85 | ND |
| RRx-001 + 7 Gy | 1.38 ± 0.20 | 0.07 ± 0.01 | 1.13 ± 0.20 | 979.00 ± 40.35 | 41.07 ± 0.82 | ND | |
| Day 7 | Vehicle +7 Gy | 0.18 ± 0.05 | 0.08 ± 0.03 | 0.07 ± 0.01 | 93.33 ± 16.29 | 30.08 ± 0.99 | 0.03 ± 0.01 |
| RRx-001 + 7 Gy | 0.21 ± 0.03 | 0.04 ± 0.00 | *0.11 ± 0.02 | *133.40 ± 16.74 | 31.36 ± 1.00 | 0.05 ± 0.01 | |
| Day 14 | Vehicle +7 Gy | 0.25 ± 0.03 | 0.18 ± 0.02 | 0.06 ± 0.01 | 78.33 ± 9.97 | 25.27 ± 0.62 | 1.62 ± 0.28 |
| RRx-001 + 7 Gy | *0.70 ± 0.20 | *0.49 ± 0.14 | *0.14 ± 0.03 | *143.25 ± 36.54 | *28.38 ± 0.55 | *4.05 ± 1.08 | |
| Day 21 | Vehicle +7 Gy | 1.19 ± 0.13 | 0.51 ± 0.08 | 0.52 ± 0.04 | 371.60 ± 66.29 | 40.40 ± 0.36 | 7.08 ± 1.59 |
| RRx-001 + 7 Gy | 1.11 ± 0.11 | 0.46 ± 0.03 | 0.50 ± 0.07 | 448.33 ± 40.37 | 39.38 ± 1.58 | 6.06 ± 1.03 | |
| Day 28 | Vehicle +7 Gy | 2.20 ± 0.34 | 0.77 ± 0.19 | 1.17 ± 0.20 | 595.50 ± 135.15 | 35.83 ± 2.18 | 2.62 ± 0.09 |
| RRx-001 + 7 Gy | 2.69 ± 0.33 | 1.18 ± 0.32 | 1.09 ± 0.11 | 497.60 ± 116.12 | 39.02 ± 1.57 | *3.66 ± 0.40 |
Values are the mean ± SEM (n = 2–3 mice/group/time point); ND, not determined. *p < 0.05 comparing the irradiated groups/time point. Significance was determined using a parametric test consisting of a general linear model ANOVA and the Kruskal–Wallis nonparametric test. **p < 0.01 comparing the nonirradiated groups combining days 2, 7, 14, 21 and 28. Significance was determined using the longitudinal mixed model repeated measures analysis. This experiment was performed in duplicate.