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. 2021 Apr 22;9:672592. doi: 10.3389/fcell.2021.672592

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Copyright © 2021 Korneev, Merriner, Gervinskas, de Marco and O’Bryan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning electron microscopy images of mouse sperm fixed using TAGA. (A) A single sperm at low magnification. (B) The head structure. The arrows mark the presence of exosome-like structures decorating the equatorial region. (C) The sperm head-tail-coupling apparatus (black arrows) where the sperm head is attached to the flagellum. The white arrows mark the presence of a second line of exosome-like structures attached to the plasma membrane. (D) The sperm mid-piece principal piece junction (white arrow). A clear impression of the helically arranged mitochondria (M) is visible on the left, and the fibrous sheath (FS) of the principal piece on the right.