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. 2021 Apr 22;9:672592. doi: 10.3389/fcell.2021.672592

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Copyright © 2021 Korneev, Merriner, Gervinskas, de Marco and O’Bryan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Focused ion beam scanning electron microscope tomography of a monolayer of mouse sperm. (A) Volume reconstruction 4.25 × 3.3 × 4.9 μm. Sperm head (green) and flagella (gray). (B) Segmented fragment of the mid-piece. (C) Virtual cross-sections (step 500 nm) of the head area. Ex, exosome-like structures attached to the plasma membrane; HTCA, head-tail-coupling apparatus; Ac, acrosome; M, mitochondria; N, highly condensed DNA within the nucleus; ODF, outer dense fibers; V, a potential nuclear vacuole. The figures are presented in orthographic projection.