Figure 4.

EuS uptake in human monocyte-derived macrophages (MDM). (A) EuS cytotoxicity in MDM after 24-hour incubation was determined by MTT assay. (B) Time course of EuS uptake of cells treated with 100 ppm Eu¹⁵³ over 24 hours as measured by UPLC-TUV. The saturation kinetic curve was fitted to experimental data using 1-site saturation curve in GraphPad Prism v7.0. (C) The mechanism of nanoparticle uptake was assessed by pre-treating with Pitstop (30 µM), Dynasore (100 µM), or CK666 (4 µM) endocytic inhibitors. Graphs depict mean ± standard error of the mean (SEM) of three biological replicates. Statistical differences were determined using two-way ANOVA between groups with a Bonferroni's post hoc-test to correct for multiple comparisons. *p < 0.05; ns: non-significant.