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. 2021 Mar 31;12(11):3154–3163. doi: 10.7150/jca.56186

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This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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Aberrant expression of miR-202-5p in colorectal cancer is critical for cell viability. A. Relative expression of miR-202-5p in 25 pairs of colorectal cancer and adjacent tissues were determined using qRT-PCR. B. Relative expression level of miR-202-5p in 5 colorectal cancer cell lines were evaluated according to normal NCM460 cell using qRT-PCR. C-D. HCT116 cells were transfected with miR-202-5p inhibitors (C) or mimics (D), then cell numbers were compared with the control group for indicated days. E-F. HCT116 cells with inhibition (E) or ectopic expression (F) of miR-202-5p were used to perform colony-formation assays and then the “ColonyArea” were analyzed by Image-J software. All data are representative of three independent experiments.