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. 2021 Apr;10(4):1743–1753. doi: 10.21037/tau-21-226

Figure 2.

Figure 2

Differentiation antagonistic non-coding RNA (DANCR) regulated the proliferation, migration, invasion, and metastasis of bladder cancer cells. (A) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) detection of DANCR siRNA transfection efficiency. **P<0.01, ***P<0.001 compare to the si-negative control (NC) group. (B) microRNA-355 (miR-335), vascular endothelial growth factor-C (VEGF-C) mRNA expression level using qRT-PCR. **P<0.01, ***P<0.001 compare to the si-NC group (C) VEGF-C expression level using Western blot**P<0.01 compare to the si-NC group. (D,E,F) si-DANCR inhibited the proliferation, migration, and invasion of bladder cancer cells, as observed by Cell Counting Kit-8, Wound healing, and Transwell assay (Crystal violet staining, Scale bar =1,000 µm, ***P<0.001 compare to the si-NC group). (G) Knockout DANCR reduced bladder cancer metastasis through endothelial cell adhesion assay. (CFDA-SE staining, Scale bar =200 µm, **P<0.01 compare to the si-NC group). OD, optical density.