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. 2021 Apr;10(4):1711–1722. doi: 10.21037/tau-20-1411

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2021 Translational Andrology and Urology. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Upregulation of METTL3 expression in TCam-2 cells is associated with cisplatin resistance. (A) m6A modification level in total RNA of TCam-2 cells and TCam-2/CDDP cells. (B,C) Relative mRNA and protein expression of METTL3 in N.C. cells, OE-METTL3 cells and si-METTL3 cells to show the transfection efficiency. GAPDH served as the internal control. The results are normalized to the expression of N.C. cells. (D) Cell viability analysis of TCam-2 cells treated with IC25 concentration of CDDP. The data are shown as the means ± S.D. *, P<0.05; **, P<0.01; ***, P<0.001. CDDP, cis-diamminedichloroplatinum II; m6A, N6-methyladenosine; S.D., standard deviation.