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. 2021 Apr;10(4):1711–1722. doi: 10.21037/tau-20-1411

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2021 Translational Andrology and Urology. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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ATG5 is a downstream target of METTL3 for autophagy regulation. (A) m6A modification level of ATG5 in TCam-2 cells upregulating or downregulating METTL3. (B,C) mRNA and protein expression of ATG5 in TCam-2 cells transfected with METTL3 plasmid or si-METTL3. GAPDH served as the internal control. (D) Immunofluorescence image of LC3-II (red) in TCam-2 cells. TCam-2 cells were treated with METTL3 transfection or si-ATG5. (E) Western blot analysis of LC3II/GAPDH ratio and Beclin1. GAPDH served as the internal control. (F) Cell viability of METTL3 overexpressing TCam-2 cells treated with or without si-ATG5 after incubated with CDDP (IC25) for 72 h. All data are shown as the means ± S.D. *, P<0.05; **, P<0.01; ***, P<0.001. m6A, N6-methyladenosine; CDDP, cis-diamminedichloroplatinum II; S.D., standard deviation.