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. 2021 Apr 20;21(6):481. doi: 10.3892/ol.2021.12742

Figure 3.

Figure 3.

Overexpression of RASA1 accelerates apoptosis and inhibits the cell cycle. (A) Relative mRNA expression of RASA1 in SW480 or SW480/OXA cells transfected with RASA1 overexpression plasmid or RASA1 siRNA. ***P<0.001 vs. vector, ###P<0.001 vs. siNC group. (B) RASA1, p21, and Ras-GTP protein expression in SW480 or SW480/OXA cells transfected with RASA1 plasmid or RASA1 siRNA. (C) Hoechst 33342 staining in SW480 or SW480/OXA cells transfected with RASA1 plasmid or RASA1 siRNA. Scale bar, 100 µm. (D) Apoptosis rate in SW480 or SW480/OXA cells following transfection with RASA1 plasmid or RASA1 siRNA. ***P<0.001 vs. vector, ###P<0.001 vs. siNC group. (E) Cell cycle distribution was analyzed by flow cytometry. ***P<0.001 vs. vector; ##P<0.01, ###P<0.001 vs. siNC. Oxa, oxaliplatin; RASA1, Ras GTPase-activating protein 1; siNC, siRNA negative control; PI, propidium iodide; FITC, fluorescein isothiocyanate.