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. 2021 Apr 20;21(6):481. doi: 10.3892/ol.2021.12742

Figure 4.

Figure 4.

Downregulation of miR-188-5p promotes SW480/OXA cell apoptosis by increasing RASA1 expression. (A) RASA1, p21, and Ras-GTP protein expression in SW480 or SW480/OXA cells following co-transfection with RASA1 plasmid or RASA1 siRNA and with miR-188-5p or inhibitor. (B) Hoechst 33342 staining in the different transfection groups. Scale bar, 100 µm. (C) Apoptosis rate in the different transfection groups. (D) Cell cycle distribution was analyzed by flow cytometry. ***P<0.001 vs. mimics + vector group; ###P<0.001 vs. inhibitor + siNC group. miR, microRNA; oxa, oxaliplatin; RASA1, Ras GTPase-activating protein 1; NC, negative control siNC, siRNA negative control; PI, propidium iodide; FITC, fluorescein isothiocyanate.