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. 2021 Apr;191(4):590–601. doi: 10.1016/j.ajpath.2021.01.001

Figure 3.

Figure 3

Inflammatory cells in the colon are increased in abundance by dextran sulfate sodium (DSS) but unchanged by enzymatically inactive tissue-type plasminogen activator (EI-tPA). A: Representative two-dimensional flow cytometry analyses of epithelial cell adhesion molecule (EpCAM)– and CD45-positive cells harvested by EDTA dissociation from mice not exposed to DSS (Control), mice exposed to 4% DSS for 5 days and then injected with vehicle (DSS), and mice exposed to DSS and then treated with EI-tPA (DSS + EI-tPA). Cells were harvested on day 12, 7 days after terminating DSS treatment. The percentages of cells identified as EpCAM- or CD45-positive, shown in the figure, are for that specific replicate. B: Percentages of isolated cells that were EpCAM- and CD45-positive are summarized. C: CD45-positive cells were further sorted to determine F4/80-positive cells. A representative study is shown. D: F4/80-positive cells are shown as percentages of the CD45-positive cell population. E: Representative two-dimensional flow cytometry analyses of EpCAM- and CD45-positive cells in colons that were re-extracted with collagenase. F: The percentages of collagenase-dissociated cells that were determined to be EpCAM- and CD45-positive are shown. Data are presented as means ± SEM (B, D, and F); n = 3 (B and F). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.