Fig. 2.

PCR verification of the insertion sites and directions of the T-DNA obtained by NGS in Pb29. a Schematic diagram of PCR primer design for verifying the insertion sites and directions of the T-DNA. LB: left border; RB: right border. b The primer combinations and product size for verifying the insertion sites and directions. Each number represents a primer combination. c The results of PCR amplification of genomic DNA of Pb29. d The results of PCR amplification of genomic DNA of poplar 741