Fig. 2.

Silencing HMGB1 partially reversed the levels of TNF-α, IL-1β, HMGB1 in LPS-induced microglia. a–c The contents of TNF-α, IL-1β, and HMGB1 in the Control, siNC, siHMGB1, LPS, LPS + siHMGB1 groups were determined by ELISA. N = 3 for each column; ^{^}P < 0.05, ^{^^^}P < 0.001 vs siNC; ^&&&P < 0.001 vs Control; ^###P < 0.001 LPS; One-way ANOVA followed by Bonferroni's post-hoc test. d QRT-PCR was used to detect the changes in the mRNA expressions of TNF-α, IL-1β, and HMGB1 in microglia in each group. β-actin was used as a control. N = 3 for each column; ^{^}P < 0.05, ^{^^}P < 0.01 vs siNC; ^&&&P < 0.001 vs Control; ^###P < 0.001 vs LPS; One-way ANOVA followed by Bonferroni's post-hoc test. Each experiment was repeated three times independently. ELISA enzyme linked immunosorbent assay, qRT-PCR quantitative reverse transcription real time polymerase chain reaction