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Sensitivity of UR-qPCR for detection of Coxiella burnetii. a The amplification curves indicate the amplification of C. burnetii recombinant DNA, serially diluted from 5.6 × 108 to 5.6 × 100 copies, denoted by numbers 8 to 0, respectively. “N” is negative control without DNA template. b Standard linear regression graph representing the relationship between initial DNA copy number and Ct value, averaged from PCR reactions performed in triplicate
