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. 2021 May 6;14:239. doi: 10.1186/s13071-021-04744-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Confirmation of Coxiella burnetii detection from tick samples. Detection curves indicate that the same five tick samples are positive for C. burnetii when tested using the CFX96 Touch Real-time PCR System (Bio-Rad). a Four samples (19M88, 19M73, 19M42, 19M22) from Gangwon Province. b One sample (19T112) from Jeju Province. “P” is the positive control using C. burnetii recombinant DNA. c Electrophoresis of PCR amplicons (295 bp long) from the five samples (19M88, 19M73, 19M42, 19M22, 19T112) positive for C. burnetii. “P” and “N” are the positive control and the negative control, without DNA template, respectively, and “M” is the 100 bp DNA marker