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. 2021 May 6;12:276. doi: 10.1186/s13287-021-02355-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Isolated EVs contain latent enzyme. After isolating at pelleting EVs from the host UMSC, the pellet was resuspended, and the suspension was measured for GALNS (a) or GUSB (b) activity. The EV-free supernatant of the isolation process contained no detectable GALNS or GUSB activities. Suspending the EVs in PBS contained a small amount of available GALNS and GUSB. Mechanical disruption by sonication increased the amount of available active GALNS enzyme. Finally, detergent-based lysis of EVs showed a significant increase in available active enzyme for both GALNS and GUSB. Error bars: mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired t test with Welch’s correction)