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. 2021 May 6;12:276. doi: 10.1186/s13287-021-02355-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — FACS scanning of EVs reveals a heterogeneous population of vesicles. UMSC-derived EVs were screened for microvesicle and exosome markers by FACS sorting. In the case of all vesicles, the EVs were separated into two populations, with the first population indicating higher side scatter than the second. Rightward peak shift indicates the presence of the stained markers. Population 1 showed a small increase in CD53+ (a), CD 151+ (b), CD14+ (c), and PECAM1+ vesicles (d). Population 2 showed a slight increase in CD151+ and CD14+ vesicles. Neither population showed an increase in CD11a+ vesicles (e). Finally, both populations had a strong presence in CD9+ vesicles (f)