Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 6;12:276. doi: 10.1186/s13287-021-02355-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Uptake of GALNS within EVs is more gradual and unaffected by M6P inhibition. Deficient fibroblasts were cultured in 2000 U GALNS (+GALNS) in the presence or absence of M6P (blue bars). This showed a strong increase in GALNS enzyme activity through the initial 24 h before declining, and this GALNS activity was inhibited by M6P. Deficient fibroblasts cultured in GALNS with an activity equal to those found in EVs (+LowGALNS) (purple bars) as well as UMSC-derived EVs (+EVs) (red bars) had a GALNS activity increase, though not significant at 24 h. Treatment of Morquio A deficient fibroblasts with UMSC-derived EVs or small amounts of GALNS showed steady slight increase up to 48 h, and this increase was not inhibited by M6P, suggesting a more gradual uptake. Deficient Morquio A fibroblasts without addition of exogenous GALNS (black bars). Error bars: mean ± SD. (*P < 0.05, ***P < 0.001, two-tailed unpaired t test with Welch’s correction)