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Isolation of EVs from higher-passage UMSC does not yield reduced GALNS activity. EVs were isolated from p6 and p10 UMSC, lysed, and measured for protein-normalized activity. The change in activity from p6 to p10 was not statistically significant (P=0.33) (a). Additionally, western blotting of lysates showed the GALNS enzyme present in both early and late passages (b). Signal normalized with actin showed equal intensity (c). Error bars: mean ± SD
