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. 2020 Oct 9;51(2):331–341. doi: 10.1002/eji.202048753

Figure 3.

Figure 3

(A) Cartoon schematic describing formulation of SPR experiments performed using LAG‐3:Fc as analyte injected over pHLA immobilized sensor chips. FC, flow cell. (B) SPR analysis of LAG‐3:Fc injection over immobilized HLA‐A*02:01 complexed with the hTERT540‐548 peptide. Sensograms reference subtracted from a blank no‐ligand control flow cell. Data are representative of two independent experimental repeats (n = 2). (C) SPR analysis showing concentration‐dependent binding (57 μM to 0.11 μM) of LAG‐3:Fc to HLA‐DR1 produced from E. coli by in vitro refolding in the presence of the HA306‐318 peptide. Data are representative of two independent experimental repeats (n = 2). (D) SPR analysis showing concentration‐dependent binding (57 μM to 0.11 μM) of LAG‐3:Fc to HLA‐DR1 covalently‐linked to CLIP and produced in sf9 insect cells. Data are representative of two independent experimental repeats (n = 2).