Table 1.

Bivalent analyte model parameters of LAG‐3:Fc binding to HLA‐DR1

Fit	[Analyte] (μM)	Rmax (RU)	RI (RU)	χ 2	kon1 (M−1 s−1)	koff1 (s−1)	kon2 (M−1 s−1)	koff2 (s−1)	KD1 (M)	KD2 (M)	KD1 (μM)
Local	7.1	1190	6.6	39.6	7.24 × 104	0.439	156.6	4.30 × 10−3	6.06 × 10−6	2.75 × 10−5	6.06
Global	0.1 – 7.1	962	4.6 – 16.9	27.6	5.22 × 104	0.380	96.9	4.10 × 10−3	7.28 × 10−6	4.23 × 10−5	7.28

[Analyte] = Concentration of injected LAG‐3:Fc analyte, RU = Response units,

R_max = Maximum analyte binding capacity in RU, RI = Bulk refractive index effect in RU,

$\chi 2=\sum \frac{{(observed-expected)}^2}{expected};$ lower χ² values indicate better model fitting.

*K_on2 was converted to M⁻¹ s⁻¹ via the following equation: k_on2 (M⁻¹ s⁻¹) = k_on2 (RU⁻¹ s⁻¹) × 100 × molecular weight of LAG‐3:Fc [44, 45].

K_D1= the affinity equilibrium dissociation constant (monovalent component).

K_D2 = the affinity equilibrium dissociation constant (bivalent component).