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. 2021 May 12;12:2760. doi: 10.1038/s41467-021-22945-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Top: Single coupling of phosphoramidites prepared on-demand to a resin-bound T₇ followed by elongation with another T₇ segment and cleavage from the resin. Bottom: Synthesis of a 51-mer using solely the on-demand approach. HPLC chromatograms of crude material: by conventional solid-phase synthesis with stored phosphoramidites (green) or with the on-demand synthesized phosphoramidites (blue). 51-mer sequence: 5′CCG CTT TCT AGT TCG TCC TCC ATA ATT AAT TTC CTA GAG TCC TAC GTG CTC 3′. B (on-demand synthesized phosphoramidite), CPG (controlled pore glass).