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. 2021 Feb 9;30(6):411–429. doi: 10.1093/hmg/ddab041

Figure 2.

Figure 2

Validation the microarray expression results using qRT-PCR. (A) Relative intensity range for gene-level summaries of qRT-PCR results indicated by boxplots for each gene in control subjects (black) and WS subjects (blue). Genes include 4 WS genes and 4 non-WS genes all show significant difference in WS compared with controls. (B) qRT-PCR of 8 genes (STX1A, LIMK1, CLIP2, GTF2I, DAPK1, CTTN, FHL3, DBN1) in un-pooled 52 subjects (34 WS, blue dots; 18 TC, black dots) except STX1A show highly significant correlations with microarray results. (C) Average fold change from pooled qRT-PCR versus array gene-level summaries of 7 WS genes (green highlight) and 21 genes outside the WS chromosome region (yellow highlight). The rank order of each gene from the gene-level test for differential expression (Supplementary Material, Table S3) is shown in parentheses.